African Journal of Biotechnology Vol. 6 (18), pp. 2130-2134, 19 common people 2007 Available online at http://www.academicjournals.org/AJB ISSN 16845315 © 2007 Academic Journals Full length look into Paper Biodiesel generation from unctuous yeast Rhodotorula glutinis with xylose assimilatory capacity Chuan-chao Dai*, Jie Tao, Feng Xie, Yi-jun Dai and Mo Zhao Jiangsu Key Laboratory for Biological mutation and Biotechnology, College of Life Science, Nanjing universal University, Nanjing, Jiangsu Province, 210046, China. Accepted 15 August, 2007 This study explored a strategy to diversify agricultural and forestry residues into microbial lipid, which could be provided transformed into biodiesel. Among the 250 yeast strains screened for xylose assimilating capacity, eight oleaginous yeasts were selected by Sudan Black B test. The lipid content of these 8 strains was determined by soxhlet beginning method. One strain (T216) was build to produce lipids up to 36 .6%, and it was identified as Rhodotorula glutinis. The best fermenting conditions were obtained as follows: glucose as carbon seed 100 g/L; yeast extract and peptone as nitrogen sources at, respectively, 8 and 3 g/L; initial pH of 5.0; inoculation volume of 5%; temperature at 28oC, shaking repair of clxxx r/min, cultivated for 96 h.
Under these conditions, R. glutinis amass lipids up to 49.25% on a cellular biomass basis and the like lipid productivity reached 14.66 g/L. Experiments with a 5-L bioreactor under the optimal socialisation conditions showed that R. glutinis accumulated lipids up to 60.69%, resulting in 23.41 g/L in lipid productivit! y. More encouraging results were observed for the lipid employment with alternative carbon sources. Corn stalk and Populus euramevicana leaves hydrolysate could be used to substitute glucose. Chemical analysis indicated that biodiesel obtained by transesterification be in possession of similar composition to that from ve stingable oil, one of the widely...If you want to get a full essay, order it on our website: OrderCustomPaper.com
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